LOWCROSS-BUFFER

비특이적 결합, 교차-반응성 및 매트릭스 효과를 최소화 하기 위한 항체 및 샘플 희석제

Antibody and sample diluent for minimizing nonspecific binding, cross-reactivities and matrix effects in immunoassays.

The formulation of LowCrossBuffer® helps to reduce interference in immunoassays. 

Thus quality of detection can be significantly improved. 

Nonspecific binding of the antibodies, negative effects of interfering substances and low or medium affinity cross-reactivities of the antibodies are minimized. 

In addition matrix effects, coming for example from blood sera or plasma specimen are reduced. 

That means that matrix effects no longer exacerbate detection of analytes. 

Minimization of all these negative effects upgrades the quality of the assay and improves the reliability of the results.

Calibration curve of an ELISA. The analyte was diluted 1:750 in PBS/BSA Tween or 1:750 in LowCross-Buffer®. The ELISA with PBS/BSA Tween shows, despite the high dilution factor, a very high coefficient of variation (error bar) due to interference. The precision of the ELISA with LowCross-Buffer® as assay diluent is significantly better.

Elimination of false positive binding in an ELISA Control of specifity in (A1-12) and blanks (H1-H12) show false positive binding. (Data from Dr. C. Specht, vivo Science GmbH, Gronau, Germany)