LOWCROSS® HRP-STAB

매트릭스 효과를 최소화하기위한 분석 희석제

Assay diluent for long-term storage of HRP conjugates and for minimizing nonspecific binding, cross-reactivities and matrix effects in immunoassays. 

LowCross® stands for superior reliability by neutralizing assay interferences including HAMAderived ones. The LowCross-effect minimizes matrix effects, crossreactivities and nonspecific binding. Thus several washing steps like in sequential assays are not necessary and one-step-incubation protocols without multiple washing can be done by using this assay diluent.

HRP-Stab gives peroxidase-antibody conjugates the crucial long-term stability over several years, even when stored as a ready-to-use solution. 

The result is an assay diluent and storage buffer for peroxidase conjugates in one solution with high reliability and convenience due to ready-to-use dilution

helf life of an HRP-labeled antibody is measured as signal (= antibody binding + enzymatic activity) in an ELISA after storage under heat stress at 45°C. The concentration of the stored antibody is 400 ng / mL.

ANTIBODY STABILIZER

2 – 8 ° C에서 단백질 또는 항체의 장기 보관을위한 안정제.

Stabilizer for long-term storage of proteins or antibodies at 2 – 8°C.

Antibody Stabilizer conserves the structure of proteins and antibodies preventing them from losing functionality due to storage.

Just dissolve proteins or antibodies with Antibody Stabilizer and store in the refrigerator.

Antibody Stabilizer is used for storage of assay antibodies and for storage of proteins, which are used as calibration standards or controls in immunoassays.

Shelf life of an antibody is measured as binding in an ELISA after  storage under heat stress at 45°C. The concentration of the stored  antibody is 400 ng/mL.

LIQUID PLATE SEALER

폴리스티렌 또는 유리 표면의 코팅 된 항체 및 항원을위한 안정제

Stabilizer for coated antibodies and antigens on polystyrene- or glasssurfaces.

After immobilization of the antibodies/ antigens and blocking, Liquid Plate Sealer® seals the plates with a uniform stabilizing layer, demonstrating good solubility and without affecting the assay afterwards.

It can also be used for stabilizing antibodies and antigens after coating on polystyrene- or glass-surfaces for protein arrays. Plates treated with Liquid Plate Sealer® can be stored for long periods after drying.

Fig. 1: BSA-Block Fig. 2: BSA-Block + Liquid Plate Sealer®

It allows long-term storage of coated plates under dry conditions even at room temperature. 

The recommended optimum storage temperature is 2 – 8°C. But most stabilized plates can also be stored dry at ambient temperature for many months without loss in activity.

Liquid Plate Sealer® can be used for stabilizing coated ELISA plates, immuno chromatographic test strips (lateral flow assays), affinity chromatography columns, protein arrays and for similar applications.

It is used as a coating stabilizer for industrial ELISA kit production.

Calibration curves of an ELISA are shown, which was either blocked only with BSA (fig. 1) or blocked with BSA and additionally stabilized with Liquid Plate Sealer® (fig. 2). Calibration curves at the starting day are identical. The calibration curve of fig. 1 after storage shows that there is no remaining binding activity of the capture antibodies.

The stabilized plate does not show significant decrease in sensitivity after 84 days of incubation at 37°C.

HRP-PROTECTORT

HRP 접합체의 장기 저장을위한 희석제

iluent for long-term storage of HRP conjugates. 

HRP-Protector™ is a long-term stabilizer for horseradish peroxidase (HRP) coupled to antibodies or Neutravidin/Streptavidin. HRP-Protector™ gives peroxidase conjugates the crucial long-term stability over several years even when stored in low concentrated ready-to-use dilutions. HRP-Protector™ can be used directly as an assay buffer for incubating the HRP conjugates. 

Typical ready-to-use concentrations for detection are between 40 – 500 ng/mL.

If you have background or interference – for example derived from cross-reactivities – we recommend using LowCross® HRP-Stab as longterm stabilizer for the conjugate. 

Shelf life of an HRP-labeled antibody is measured as signal (= antibody binding + enzymatic activity) in an ELISA after storage under heat stress at 45°C. The concentration of the stored antibody is 400 ng/mL.