SAMPLE BUFFER

면역 분석을위한 샘플 및 항체 희석 완충액

Sample and antibody dilution buffer for immunoassays.

Sample Buffer is designed for trouble free assays, which are not affected by interference derived from samples.

If you have background or false positives – for example derived from cross-reactivities or matrix effects – we recommend using LowCross- Buffer® as the assay diluent.

STRIPPING BUFFER

다중 리프로빙을 위해 막에서 항체를 제거하기 위한 버퍼

Buffer for stripping antibodies from membranes for multiple reprobing. 

Stripping Buffer removes reaction solution and primary and secondary  antibodies from Western blotting membranes.

After stripping, the membrane can be used for repeated detection (reprobing) with antibodies.

Stripping Buffer does not contain mercapto-ethanol nor DTT.

COATING BUFFER 10×

표면에 단백질 및 항체를 흡착 고정시키기위한 완충액

Buffer for adsorptive immobilization of proteins and antibodies on plastic  surfaces (for example microtiter plates)  or other protein binding surfaces.

Depending on the capture protein or the capture antibody the pH of the Coating Buffer is crucial for efficient immobilization. Select from Coating Buffer pH 7.4 and pH 9.6, as the pHvalue can have an influence on the steric structure of proteins or antibodies, thus affecting their immobilization.

For the optimization of the coating procedure of a newly developed immunoasay we strongly recommend 

Washing steps are needed to remove unbound and excessive components which are able to interfere with the assay. 

Washing Buffer is optionally available based on TRIS or PBS.

Washing Buffer is suitable for automatic washers (Please note the specifications of your device and follow the instruction manual of your washer!). 

testing the two Coating Buffers side by side. Depending on the surface as well as on proteins or antibodies the required incubation time can vary. Consequently, individual optimization of the incubation procedure is recommended.

10× concentrate – simply dilute with water!

WASHING BUFFER 10×

TRIS 또는 PBS에 기초한 세척 완충액

Washing steps are needed to remove unbound and excessive components which are able to interfere with the assay. 

Washing Buffer is optionally available based on TRIS or PBS.

Washing Buffer is suitable for automatic washers (Please note the specifications of your device and follow the instruction manual of your washer!). 

For immunohistochemistry (IHC) we offer Washing Buffer without Tween and detergents. 

10× concentrate – simply dilute with water!

Washing steps are needed to remove unbound and excessive components which are able to interfere with the assay. 

Washing Buffer is optionally available based on TRIS or PBS.

Washing Buffer is suitable for automatic washers (Please note the specifications of your device and follow the instruction manual of your washer!). 

For immunohistochemistry (IHC) we offer Washing Buffer without Tween and detergents. 

10× concentrate – simply dilute with water!